| 產(chǎn)品名稱 |
5H8 |
| 商品貨號(hào) |
B163798 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinoma |
| Applications |
The antibody reacts with periostin, a secreted protein. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. |
| Clinical Data |
The levels of serum periostin may be an indicator of tumor invasion and progression in patients with thymoma and nonsmall cell lung carcinomas (NSCLC). |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against periostin |
| Cellular Products |
immunoglobulin; monoclonal antibody; against periostin |
| Comments |
The antibody reacts with periostin, a secreted protein. The levels of serum periostin may be an indicator of tumor invasion and progression in patients with thymoma and nonsmall cell lung carcinomas (NSCLC). Animals were immunized with Fc-TCG1, which is similar to periostsin. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Note: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. |
| Cryopreservation |
Freeze medium: A 1:1 mixture of culture medium and fetal bovine serum, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
LB Chen |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Sasaki H, et al. Serum level of the periostin, a homologue of an insect cell adhesion molecule, in thymoma patients. Cancer Lett. 172: 37-42, 2001. PubMed: 11595127
Sasaki H, et al. Serum level of the periostin, a homologue of an insect cell adhesion molecule, as a prognostic marker in nonsmall cell lung carcinomas. Cancer 92: 843-848, 2001. PubMed: 11550156
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