| 產(chǎn)品名稱 |
A3 |
| 商品貨號(hào) |
B163872 |
| Organism |
Homo sapiens, human |
| Cell Type |
T lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute T cell leukemia |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The A3 subclone was derived from a Jurkat cell line obtained from the laboratory of Gerald Crabtree at Stanford University.
|
| Clinical Data |
male |
| Comments |
The Jurkat cells were treated with Fas Antibody and isolated by limiting dilution to obtain a cell line that had a low spontaneous rate of resistance to Fas-medicated apoptosis.
The resulting wild-type A3 subclone is very sensitive to Fas-mediated apoptosis.
Wild-type A3 cells were made neomycin resistant and treated with three cycles of exposure to the frameshifting mutagen ICR-191 to isolate clones harboring recessive mutations that were resistant to killing by Fas antibody.
ICR-191 treated clones were serially diluted in 96-well plates in the presence of Fas Antibody for 3 to 5 weeks.
Two of these ICR-191 treated clones have been deposited at the ATCC. They are I 9.2 (ATCC CRL-2571), a clone with a mutation in the cysteine protease caspase-8/FLICE and I 2.1 (ATCC CRL-2572), a clone with a mutation in the adaptor FADD. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 2 x 105 and 2 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
|
| Cryopreservation |
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,11 D16S539: 11 D5S818: 9 D7S820: 8,10 THO1: 6,9.3 TPOX: 8,10 vWA: 17,18 |
| Name of Depositor |
J Blenis |
| Deposited As |
human |
| References |
Juo P, et al. Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade. Curr. Biol. 8: 1001-1008, 1998. PubMed: 9740801
Juo P, et al. FADD is required for multiple signaling events downstream of the receptor Fas. Cell Growth Differ. 10: 797-804, 1999. PubMed: 10616904
Juo P, et al. Fas activation of the p38 mitogen-activated protein kinase signalling pathway requires ICE/CED-3 family proteases. Mol. Cell. Biol. 17: 24-35, 1997. PubMed: 8972182
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