| 產(chǎn)品名稱 | A-72 |
|---|---|
| 商品貨號 | B163892 |
| Organism | Canis familiaris, dog |
| Tissue | unknown |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | tumor |
| Age | 8 years |
| Gender | female |
| Strain | Golden retriever |
| Applications | efficacy testing Ref |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation | The line was established from a 1 cm diameter tumor taken from the left thigh of a female Golden Retriever. |
| Clinical Data | female |
| Virus Susceptibility | Canine adenovirus 2
Canine coronavirus , Canine coronavirus Canine parainfluenza virus Canid herpesvirus 1 Canine distemper virus Canine minute virus |
| Comments | A canine parvovirus related to Feline panleukopenia virus has been recovered from A-72 cells. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing | Remove medium, rinse with fresh 0.25% trypsin, 0.53 mM EDTA solution, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation | Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 100%
Temperature: 37°C |
| Name of Depositor | LN Binn |
| Deposited As | Canis familiaris |
| References | Binn LN, et al. Establishment of a canine cell line: derivation, characterization, and viral spectrum. Am. J. Vet. Res. 41: 855-860, 1980. PubMed: 6254399 The line was established from a 1 cm diameter tumor taken from the left thigh of a female Golden Retriever. Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.