2018国产大陆天天弄,亚洲欧美日韩久久精品第一区,日韩精品久久久久久免费,国产精品国产三级国产av品爱网,妺妺窝人体色WWW聚色窝仙踪,亚洲av无码专区国产乱码电影,欧美性猛交XXXX乱大交3,无码人妻h动漫

The cells are resistant to 0.1 mM 6-thioguanine.

Unlike the parental line, these cells are sensitive to HAT.

Tested and found negative for ectromelia virus (mousepox).

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10⁵ cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ cells/mL.

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Kohler G, et al. Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7: 758-761, 1977. PubMed: 563329

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Goldsby RA, et al. Hybrid cell lines with T-cell characteristics. Nature 267: 707-708, 1977. PubMed: 301614

Hammerling GJ. T lymphocyte tissue culture lines produced by cell hybridization. Eur. J. Immunol. 7: 743-746, 1977. PubMed: 304002

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.