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G-3-5
G-3-5
規(guī)格:
貨期:
編號(hào):B164481
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 G-3-5
商品貨號(hào) B164481
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Autopsy
Applications
It is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized human brain homogenate of multiple sclerosis plaques obtained at autopsy.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against alpha actinin
Cellular Products
immunoglobulin; monoclonal antibody; against alpha actinin
Comments
Animals were immunized human brain homogenate of multiple sclerosis plaques obtained at autopsy.
Spleen cells were fused with NS-1 myeloma cells.
The antibody recognizes a novel intermediate filament associated protein (MW 100000) with similarity to alpha actinin.
This antibody intensely stains astroglia and related macroglia in cryostat sections of human and rat central nervous system tissue.
It is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cultures at a cell concentration between 0.5 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgM
Name of Depositor SK Malhotra
Deposited As mouse (B cell); mouse (myeloma)
References

Jeffcoat S, et al. A novel intermediate filament-associated protein: further characterization of the G.3.5 antigen. Cytobios 82: 81-99, 1995. PubMed: 7587376

. . Ann. N.Y. Acad. Sci. 436: 492-494, 1984.

Price KA, et al. Localization and characterization of an intermediate filament- associated protein. Cytobios 76: 157-173, 1993. PubMed: 8181306

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