| 產(chǎn)品名稱 |
IgG-3B2 |
| 商品貨號(hào) |
B164841 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with a bacterially produced fusion protein expressing pGST-dSREBP (amino acids 1 to 451). It can be used in immunoblotting assays to detect Drosophila SREBP and to study SREBP in S2 cells grown in various conditions. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Animals were immunized with a bacterially produced fusion protein expressing pGST-dSREBP (amino acids 1 to 451). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Drosophila sterol regulatory element binding protein (SREBP) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Drosophila sterol regulatory element binding protein (SREBP) |
| Comments |
Animals were immunized with a bacterially produced fusion protein expressing pGST-dSREBP (amino acids 1 to 451). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody recognizes both the precursor and mature forms of the Drosophila SREBP transcription factor in cultured S2 (Schneider) Drosophila cells. It can be used in immunoblotting assays to detect Drosophila SREBP and to study SREBP in S2 cells grown in various conditions. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml. Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse IgG1 kappa |
| Name of Depositor |
YK Ho, JL Goldstein |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Seegmiller AC, et al. The SREBP pathway in Drosophila: regulation by palmitate, not sterols. Dev. Cell 2: 229-238, 2002. PubMed: 11832248
Dobrosotskaya IY, et al. Regulation of SREBP processing and membrane lipid production by phospholipids in Drosophila.. Science 296: 879-883, 2002. PubMed: 11988566
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