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pWS12
pWS12
規(guī)格:
貨期:
編號(hào):B237754
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pWS12
商品貨號(hào) B237754
Designations pWS12
GenBank Number

M11133

Species Wound tumor virus
Depositors DL Nuss
Vector
Construct size (kb): 3.72000002861023
Insert
DNA: cDNA
Insert lengths(kb): 0.8500000238418579
Gene product: segment S12, Pns12 polypeptide
Insert Size (kb) 0.850
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains internal HindIII and AccI sites.
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.90; EcoRI--3.8; PstI--3.8; HindIII--3.0, 0.87; XbaI--3.8.
Linearize the plasmid with AvaI for in vitro transcription.
This is a full-length clone of virus segment S12.
The cDNA ends were tailored to terminal BamHI sites before cloning into the BamHI site of pGEM-1.
Classification Phytoreovirus
References

Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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