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LBRM TG6
LBRM TG6
規(guī)格:
貨期:
編號:B164962
品牌:Mingzhoubio

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產品名稱 LBRM TG6
商品貨號 B164962
Organism Mus musculus, mouse
Cell Type T lymphocyte; radiation induce
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoma
Strain B10.BR
Applications
The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma (see ATCC TIB-155).
The cells produce IL-2 in response to IL-1 and PHA.
These cells can be used to measure IL-1 activity in a very rapid and sensitive assay.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma (see ATCC TIB-155).
Receptor Expression
interleukin 1 (IL-1)
Genes Expressed
interleukin 2; IL-2; interleukin-2
Cellular Products
interleukin 2; IL-2; interleukin-2
Comments
The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma (see ATCC TIB-155).
The cells produce IL-2 in response to IL-1 and PHA.
These cells can be used to measure IL-1 activity in a very rapid and sensitive assay.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Iscove's modified Dulbecco's medium with 0.02 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 5 cells/mL and maintain between 1 x 10 5 and 1 x 10 6 cells/mL.

Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor JW Larrick
Deposited As Mus musculus
References

Larrick JW, et al. An improved assay for the detection of interleukin 1. J. Immunol. Methods 79: 39-45, 1985. PubMed: 3873500

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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