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The tissue donor suffered from Marfan syndrome and floppy mitral valve syndrome.

female

The tissue donor suffered from Marfan syndrome and floppy mitral valve syndrome.

There was no prior family history of Marfan syndrome.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 8 to 10 days.