| 產(chǎn)品名稱(chēng) |
MBL-Sm-1A6 |
| 商品貨號(hào) |
B165094 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for one of the two major epitopes on S. mansoni paramyosin that predominate in the in vivo immune response to the parasite. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with a partially purified S. mansoni antigen preparation plus BCG. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against paramyosin from Schistosoma mansoni |
| Cellular Products |
immunoglobulin; monoclonal antibody; against paramyosin from Schistosoma mansoni |
| Comments |
Animals were immunized with a partially purified S. mansoni antigen preparation plus BCG. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody is specific for one of the two major epitopes on S. mansoni paramyosin that predominate in the in vivo immune response to the parasite. The epitope recognized is distinct from that recognized by MBL-Sm-4B1 (ATCC HB-193). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
A Sher, EJ Pearce |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Lanar DE, et al. Identification of paramyosin as schistosome antigen recognized by intradermally vaccinated mice. Science 234: 593-596, 1986. PubMed: 3094144
Pearce EJ, et al. Immunochemical characterization and purification of Sm-97, a Schistosoma mansoni antigen monospecifically recognized by antibodies from mice protectively immunized with a nonliving vaccine. J. Immunol. 137: 3593-3600, 1986. PubMed: 2431037
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