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NCI-H1385 [H1385]
NCI-H1385 [H1385]
規(guī)格:
貨期:
編號(hào):B165260
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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產(chǎn)品名稱 NCI-H1385 [H1385]
商品貨號(hào) B165260
Organism Homo sapiens, human
Tissue lung; derived from metastatic site: lymph node
Product Format frozen
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease stage 3A, carcinoma; non-small cell lung cancer
Age 49 years
Gender female
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was established in April 1986 from lymph node, a metastatic site.
Clinical Data
49 years old
female
Black
The patient was a smoker, 33 packs year.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium.
Medium Renewal: 2 times per week
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818: 13
D7S820: 8,10
THO1: 7
TPOX: 8,9
vWA: 17,19
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
Year of Origin 1986
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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