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The tumor developed in a patient with a 75 packs-year history of cigarette smoking.

• 0.005 mg/ml Insulin (Sigma I1882)
• 0.01 mg/ml Transferrin (Sigma T1147)
• 30nM Sodium selenite (final conc.) (Sigma S9133) 
• 10 nM Hydrocortisone (final conc.) (Sigma H0135)
• 10 nM beta-estradiol (final conc.) (Sigma E2557)  
• extra 2mM L-glutamine (for final conc. of 4.5 mM) (Sigma G5783) 
• 5% fetal bovine serum (ATCC 30-2020)

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.