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NCI-H211 [H211]
NCI-H211 [H211]
規(guī)格:
貨期:
編號(hào):B165314
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 NCI-H211 [H211]
商品貨號(hào) B165314
Organism Homo sapiens, human
Tissue lung
Product Format frozen
Culture Properties aggregate in suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease stage E, carcinoma; small cell lung cancer
Age 50 years
Gender female
Ethnicity Caucasian, White
Storage Conditions liquid nitrogen vapor phase
Karyotype iso(3p), t(3;4)(pter-q12), t(3;11)(qter-p25)
Derivation
The line was established in January 1980.
Clinical Data
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker. 35 pack years.

Comments
Fast growing.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cell clusters which should not be broken up (the cells lose viability when aggregates are broken up).
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 13
D5S818: 11
D7S820: 10,12
THO1: 6
TPOX: 11
vWA: 14,18
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
Year of Origin 1980
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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