2018国产大陆天天弄,亚洲欧美日韩久久精品第一区,日韩精品久久久久久免费,国产精品国产三级国产av品爱网,妺妺窝人体色WWW聚色窝仙踪,亚洲av无码专区国产乱码电影,欧美性猛交XXXX乱大交3,无码人妻h动漫

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > WR21
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
WR21
WR21
規(guī)格:
貨期:
編號:B165958
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 WR21
商品貨號 B165958
Organism Mus musculus, mouse
Tissue submandibular salivary gland
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease adenocarcinoma
Age adult
Gender male
Strain subline 69-2 (C57BL/6, SJL transgenic)
Applications
WR21 cells were cultured from a salivary tumor taken from an adult male wap-ras subline 69=2 transgenic mouse in the fall of 1989.
Clinical Data
WR21 cells were cultured from a salivary tumor taken from an adult male wap-ras subline 69=2 transgenic mouse in the fall of 1989.
male
Oncogene c-H-ras + (human)
Genes Expressed
vimentin; fibronectin,c-H-ras + (human)
Cellular Products
vimentin; fibronectin
Tumorigenic Yes
Effects
Yes, the cells give rise to aggressive, highly anaplastic solid tumors when injected subcutaneously into athymic nude mice
Comments
WR21 cells were cultured from a salivary tumor taken from an adult male wap-ras subline 69=2 transgenic mouse in the fall of 1989.
These mice express an activated human c-Ha-ras gene and develop adenocarcinomas arising from the submandibular gland and/or mammary progenitor cells by one year of age.
Subline 69-2 has a random bred genetic background of two inbred mouse strains, C57BL/6 and SJL.
The cells were subcloned in 1990.
It is impossible to ascertain the original cell type with any certainty.
They express fibronectin and vimentin but not cytokeratin or desmin.
WR21 cells have an activated human Ha-ras oncogene on the Y chromosome and express Asp-12 mutant ras protein.
In nude mice with WR21 tumors, approximately 90% had lung metastases by the fifth week of tumor growth, but no tumors metastasized to the salivary gland.
WR21 tumor growth was inhibited by cyclophosphamide, 5-fluorouracil, adriamycin, mitomycin C and actinomycin D but not methotrexate.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor LL Nielsen
Deposited As Mus musculus
References

Nielsen LL, et al. Development of a nude mouse model of ras-mediated neoplasia using WR21 cells from a transgenic mouse salivary tumor. In Vivo 8: 295-302, 1994. PubMed: 7803707

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
金溪县| 齐河县| 泉州市| 永德县| 威宁| 景东| 玉山县| 岳池县| 银川市| 建阳市| 江阴市| 阿合奇县| 辉县市| 安康市| 大冶市| 太仆寺旗| 闻喜县| 抚松县| 兰西县| 彭州市| 余姚市| 五台县| 白城市| 筠连县| 西平县| 慈利县| 密云县| 金堂县| 黄平县| 澄江县| 合川市| 大化| 凉山| 谷城县| 平顶山市| 涞水县| 拜城县| 成安县| 黄平县| 漳州市| 平湖市|