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WR21
WR21
規(guī)格:
貨期:
編號:B165958
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 WR21
商品貨號 B165958
Organism Mus musculus, mouse
Tissue submandibular salivary gland
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease adenocarcinoma
Age adult
Gender male
Strain subline 69-2 (C57BL/6, SJL transgenic)
Applications
WR21 cells were cultured from a salivary tumor taken from an adult male wap-ras subline 69=2 transgenic mouse in the fall of 1989.
Clinical Data
WR21 cells were cultured from a salivary tumor taken from an adult male wap-ras subline 69=2 transgenic mouse in the fall of 1989.
male
Oncogene c-H-ras + (human)
Genes Expressed
vimentin; fibronectin,c-H-ras + (human)
Cellular Products
vimentin; fibronectin
Tumorigenic Yes
Effects
Yes, the cells give rise to aggressive, highly anaplastic solid tumors when injected subcutaneously into athymic nude mice
Comments
WR21 cells were cultured from a salivary tumor taken from an adult male wap-ras subline 69=2 transgenic mouse in the fall of 1989.
These mice express an activated human c-Ha-ras gene and develop adenocarcinomas arising from the submandibular gland and/or mammary progenitor cells by one year of age.
Subline 69-2 has a random bred genetic background of two inbred mouse strains, C57BL/6 and SJL.
The cells were subcloned in 1990.
It is impossible to ascertain the original cell type with any certainty.
They express fibronectin and vimentin but not cytokeratin or desmin.
WR21 cells have an activated human Ha-ras oncogene on the Y chromosome and express Asp-12 mutant ras protein.
In nude mice with WR21 tumors, approximately 90% had lung metastases by the fifth week of tumor growth, but no tumors metastasized to the salivary gland.
WR21 tumor growth was inhibited by cyclophosphamide, 5-fluorouracil, adriamycin, mitomycin C and actinomycin D but not methotrexate.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor LL Nielsen
Deposited As Mus musculus
References

Nielsen LL, et al. Development of a nude mouse model of ras-mediated neoplasia using WR21 cells from a transgenic mouse salivary tumor. In Vivo 8: 295-302, 1994. PubMed: 7803707

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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