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X16C8.5 [X16C]
X16C8.5 [X16C]
規(guī)格:
貨期:
編號(hào):B165964
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 X16C8.5 [X16C]
商品貨號(hào) B165964
Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease reticulum cell sarcoma
Strain BALB/cAnN
Applications
Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Karyotype near tetraploid; modal number = 78; range = 39 to 84
Derivation
Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.
Antigen Expression
I-A+; H-2d
Receptor Expression
Fc
Genes Expressed
immunoglobulin (both surface and secreted, sIg+),I-A+; H-2d,Expression of I-A and H-2 is increased by treatment with interferon.
Cellular Products
immunoglobulin (both surface and secreted, sIg+)
Tumorigenic No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.
Expression of I-A and H-2 is increased by treatment with interferon.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Isotype IgM
Population Doubling Time 18 hrs
Name of Depositor KJ Kim
Deposited As Mus musculus
References

Kim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843

Kim KJ, et al. Characterization of T-cell-soluble factors modulating the expression of Ia and H-2 antigens on BALB/c B lymphoma cell lines. Cell. Immunol. 76: 253-267, 1983. PubMed: 6404559

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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