| 產(chǎn)品名稱 |
Rat coronavirus |
| 商品貨號 |
B226363 |
| Classification |
Nidovirales, Coronaviridae, Coronavirus,Group 2 |
| Deposited As |
Rat Coronavirus |
| Agent |
Rat coronavirus |
| Strain |
8190 |
| Common Name |
Rat coronavirus |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Lungs of rat |
| Product Format |
frozen |
| Storage Conditions |
?Store at -70°C or colder |
| Comments |
Antigenic cross reactivity with mouse coronavirus. Rats inoculated with this material (mouse brain stock) do not seroconvert to other agents. VR-1410 and VR-635 are the same strain provided by different contributors. Antigenic cross reactivity with mouse coronavirus. Rats inoculated with this material (mouse brain stock) do not seroconvert to other agents. |
| Effect on Host |
Yes, in vitro effects: Causes large syncytia in L2 or PRK cells Yes, in vivo effects: respiratory distress and death in suckling rats |
| Recommended Host |
L2 cells (ATCC CCL-149) Recommended Host: ?L2 (Percy) (ATCC? CCL-81?) rat; mouse; L2 cells; primary rat kidney cells |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and rinse cell sheet with 1X DPBS. Remove 1X DPBS and then inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.01. Adsorb for 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 2 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture. |
| Name of Depositor |
DJ Gaertner |
| Special Collection |
ATCC |
| Source |
Lungs of rat |
| References |
Parker JC, et al. Rat coronavirus (RCV): a prevalent, naturally occurring pneumotropic virus of rats. Arch. Gesamte Virusforsch. 31: 293-302, 1970. PubMed: 4099196
|
