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UACC-462
UACC-462
規(guī)格:
貨期:
編號:B227987
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 UACC-462
商品貨號 B227987
Organism Homo sapiens, human
Tissue pancreas; derived from metastatic site: peritoneal mass
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease pancreatic cancer
Age 41
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images ATCC CRL-2989 Cell Micrograph
Derivation UACC-462 cell line was derived from a 41 year-old male with pancreatic cancer metastatic to the peritoneal mass.
Clinical Data 41 years
male
Caucasian
Complete Growth Medium M-41 medium. The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium (final conc.):
  • fetal bovine serum to a final concentration of 5%
  • 0.01 mg/ml transferrin
  • 0.01 mg/ml insulin
  • 5 µg/ml (55 U/ml) catalase
  • 3.6 µg/ml (0.01 mM) hydrocortisone
  • 10 ng/ml EGF
  • 3 ng/ml Estradiol
  • 70 µg/ml O-phosphorylethanilamine
  • 0.0008 µg/ml L-thyroxine
  • extra 2mM L-glutamine
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of solutions for culture vessels of other sizes.


1. Remove and discard culture medium.

2. Briefly rinse the cell layer with 5.0 ml of Ca++/Mg++ free Dulbecco's phosphate-buffered saline (DPBS) to remove all traces of serum which contains trypsin inhibitor.

3. Add 5.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4. Add 5.0 ml of complete growth medium and aspirate cells by gently pipetting.

5. Transfer all cell suspension to a 15ml centrifuge tube and spin at approximately 125 x g for 10 minutes. Discard supernatant.

6. Re-suspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. 


An inoculum of 3.0 X 104 to 5.0 X 104 viable cells/cm2 is recommended.
Incubate cultures at 37.0°C.

Cryopreservation Freeze medium: Complete growth medium supplemented with 10% (v/v) FBS and 10% (v/v) DMSO. 

Storage temperature: liquid nitrogen vapor phase

Culture Conditions Atmosphere: air, 100%.

Temperature: 37°C

Cells per Vial ≥ 1.0 x 10^6
STR Profile TH01: 9, 9.3
D5S818: 12, 13
D13S317:  9, 12
D7S820: 12
D16S539: 13
CSF1PO: 10, 12
Amelogenin: X, Y
vWA: 17
TPOX: 8, 10
Name of Depositor Kathy Brown
Passage History 38
Year of Origin 1985
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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