2018国产大陆天天弄,亚洲欧美日韩久久精品第一区,日韩精品久久久久久免费,国产精品国产三级国产av品爱网,妺妺窝人体色WWW聚色窝仙踪,亚洲av无码专区国产乱码电影,欧美性猛交XXXX乱大交3,无码人妻h动漫

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > lambdaDL10
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
lambdaDL10
lambdaDL10
規(guī)格:
貨期:
編號:B228019
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 lambdaDL10
商品貨號 B228019
Designations lambdaDL10
Depositors B Windle
Biosafety Level 1
Vector Information
Size (kb): 39.0000000000000000
Vector: lambdaDL10 (phage, lambda - replacement)
Promoters: Promoter lac
Construction: pUC9, lambdaD69, M13mp10
Construct size (kb): 39.0
Features: insert detection: lacZ'
promoter: lac
replicon: lambda
terminator: none
enhancer: none
Applications
expression vector
vector for constructing cDNA libraries
vector permitting construction of fusion proteins
Comments
A BamHI digest removes a 930 bp fragment containing the lacZalpha coding frame. Thus use of BamHI as a cloning site and selection of clear plaques on Xgal media may not indicate the presence of a recombinant insert.
Escherichia coli JM109 is not a good host for this vector.
If HindIII, SacI, XbaI, XhoI, or BamHI are used as cloning sites, the vector can accept fragments of 0 - 12 kb. Inserts in HindIII, SacI, XbaI, BamHI, and SalI inactivate lacZ.
If SalI is used as a cloning site, the vector can accept fragments of 9 - 20 kb. Replacement of the SalI fragment with a DNA insert results in a lambdacIII- phage that forms virtually clear plaques.
A 435 bp HaeII fragment of pUC9, encoding beta-galactosidase, was inserted into the HindIII site of lambdaD69 to produce lambdaDL9. The multiple cloning sites of M13mp10 were crossed into lambdaDL9 to produce lambdaDL10.
lambdaDL10 (ATCC 37489) and lambdaDL11 (ATCC 37490) have opposite orientations of the restriction sites within lacZ.
References

Windle BE. Phage lambda and plasmid expression vectors with multiple cloning sites and lacZ alpha-complementation. Gene 45: 95-99, 1986. PubMed: 2946626

Brad Windle, personal communication

Shipped freeze-dried
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
类乌齐县| 南平市| 札达县| 南平市| 建昌县| 罗山县| 大理市| 吉首市| 驻马店市| 莆田市| 图木舒克市| 河北省| 海丰县| 滨海县| 达日县| 安仁县| 南昌县| 岗巴县| 长沙市| 江北区| 禹州市| 海宁市| 江永县| 游戏| 应用必备| 南投市| 清镇市| 兰州市| 秦皇岛市| 石嘴山市| 义乌市| 新闻| 金坛市| 来安县| 太仆寺旗| 明光市| 登封市| 化德县| 南乐县| 尖扎县| 咸宁市|