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lambdaDL10
lambdaDL10
規(guī)格:
貨期:
編號:B228019
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 lambdaDL10
商品貨號 B228019
Designations lambdaDL10
Depositors B Windle
Biosafety Level 1
Vector Information
Size (kb): 39.0000000000000000
Vector: lambdaDL10 (phage, lambda - replacement)
Promoters: Promoter lac
Construction: pUC9, lambdaD69, M13mp10
Construct size (kb): 39.0
Features: insert detection: lacZ'
promoter: lac
replicon: lambda
terminator: none
enhancer: none
Applications
expression vector
vector for constructing cDNA libraries
vector permitting construction of fusion proteins
Comments
A BamHI digest removes a 930 bp fragment containing the lacZalpha coding frame. Thus use of BamHI as a cloning site and selection of clear plaques on Xgal media may not indicate the presence of a recombinant insert.
Escherichia coli JM109 is not a good host for this vector.
If HindIII, SacI, XbaI, XhoI, or BamHI are used as cloning sites, the vector can accept fragments of 0 - 12 kb. Inserts in HindIII, SacI, XbaI, BamHI, and SalI inactivate lacZ.
If SalI is used as a cloning site, the vector can accept fragments of 9 - 20 kb. Replacement of the SalI fragment with a DNA insert results in a lambdacIII- phage that forms virtually clear plaques.
A 435 bp HaeII fragment of pUC9, encoding beta-galactosidase, was inserted into the HindIII site of lambdaD69 to produce lambdaDL9. The multiple cloning sites of M13mp10 were crossed into lambdaDL9 to produce lambdaDL10.
lambdaDL10 (ATCC 37489) and lambdaDL11 (ATCC 37490) have opposite orientations of the restriction sites within lacZ.
References

Windle BE. Phage lambda and plasmid expression vectors with multiple cloning sites and lacZ alpha-complementation. Gene 45: 95-99, 1986. PubMed: 2946626

Brad Windle, personal communication

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