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pBR0.9EZZ [probe C]
pBR0.9EZZ [probe C]
規(guī)格:
貨期:
編號:B228424
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pBR0.9EZZ [probe C]
商品貨號 B228424
Designations pBR0.9EZZ [probe C]
Species Homo sapiens, human
Depositors M Lefranc
Vector
Construct size (kb): 3.599999904632568
Insert
DNA: genomic
Insert lengths(kb): 0.8999999761581421
Gene product: immunoglobulin heavy chain gamma [IGHG@*]
Insert Size (kb) 0.900
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.7, 0.9; EcoRI--2.7, 0.9; HindIII--3.3, 0.3; BamHI--3.6; EcoRI/HindIII--2.55, 0.61, 0.41, 0.3.
The probe detects a 9.7 kb BamHI genomic fragment, a 6.5 kb and 8.9 kb HindIII genomic fragment, and a 9.2 SacI genomic fragment.
This was isolated from lambdaEZZ from a region about 13 kb 3' to IGHG3. This region and similar ones downstream of IGHG genes appear to be highly homologous spots of recombination involved in multigene deletions of the IGH constant region locus.
References

Keyeux G, et al. A multigene deletion in the human IGH constant region locus involves highly homologous hot spots of recombination. Genomics 5: 431-441, 1989. PubMed: 2613231

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