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D13-2
D13-2
規(guī)格:
貨期:
編號(hào):B229141
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) D13-2
商品貨號(hào) B229141
Designations D13-2
GenBank Number

M28561

Species Mus musculus, mouse
Depositors DW Sears
Applications
in another host, produces protein Fc gamma high affinity receptor (IgG2a)
Vector
Construct size (kb): 4.311999797821045
Insert
DNA: cDNA
Insert lengths(kb): 1.279000043869019
Tissue: macrophage J774 cell line
Gene product: Fc gamma high affinity receptor (IgG2a) [Fcr1]
Target Gene: Fc gamma high affinity receptor (IgG2a)
Insert Size (kb) 1.279
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.6, 0.6, 0.1; BamHI--3.0, 1.4; BglI--2.8, 1.3, 0.3; HindIII--4.35; SacI--3.8, 0.5.
Restriction digest of the clone give the following sizes (kb): PstI--3.6, 0.6; BamHI--3.0, 1.4; BglI--2.8, 1.3, 0.3; HindIII--4.35; SacI--3.8, 0.5.
The cDNA detects a single genomic HindIII fragment (8.8 kb), a doublet of EcoRI fragments (4.3 kb) and several TaqI, PvuII, BamHI, and BclI fragments.
The insert contains a complete open reading frame of 1212 nucleotides, one nucleotide 5' untranslated (C), and 61 nucleotides 3' untranslated followed by AAAAA.
The vector is a derivative of pcD with added restriction sites (BamHI, XmaI SmaI KpnI SacI) and a T3 promoter 3' of the insert site.
When excised with BamHI, the insert will contain 5' and 3' vector sequences. The BamHI sites are at positions 414 and 541 of the vector and the PstI site is at 505.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Sears DW, et al. Molecular cloning and expression of the mouse high affinity Fc receptor for IgG1. J. Immunol. 144: 371-378, 1990. PubMed: 2136886

Duane W Sears, personal communication

梅經(jīng)理 17280875617 1438578920
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沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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